Coding

Part:BBa_J92008:Design

Designed by: Timothy Fenton   Group: UC Davis - BIM189C - S10   (2010-05-31)

M. Ruminantium RNA polymerase Subunit B"


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1277
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 792
    Illegal BglII site found at 1116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to make silent mutations at 775, 853, 1030, to get rid of PstI sites.

Source

From genomice

References